Dextrans and Glycogens as Particulate Tracers for Studying Capillary Permeability
نویسندگان
چکیده
Commercially available glycogens and dextrans can be used as biological particulate tracers in work on capillary permeability. These polysaccharides are well tolerated in intravenous injection and induce no vascular leakage when applied topically (cremaster test) in mice and in Wistar-Furth rats. The particles stain adequately with lead after aldehyde-OsO(4) fixation in phosphate buffer and provide a relatively wide set of probes ( approximately 45 A-300 A) for work on the large and small pore systems.
منابع مشابه
PERMEABILITY OF INTESTINAL CAPILLARIES Pathway followed by Dextrans and Glycogens
The pathway followed by macromolecules across the wall of visceral capillaries has been studied by using a set of tracers of graded sizes, ranging in diameter from 100 A (ferritin) to 300 A (glycogen) . Polysaccharide particles, i .e. dextran 75 (mol wt -75,000 ; diam -125 A), dextran 250 (mol wt 250,000 ; diam -225 A), shellfish glycogen (diam -200 A) and rabbit liver glycogen (diam -300 A), a...
متن کاملPermeability of Intestinal Capillaries
The pathway followed by macromolecules across the wall of visceral capillaries has been studied by using a set of tracers of graded sizes, ranging in diameter from 100 A (ferritin) to 300 A (glycogen). Polysaccharide particles, i.e. dextran 75 (mol wt approximately 75,000; diam approximately 125 A), dextran 250 (mol wt 250,000; diam approximately 225 A), shellfish glycogen (diam approximately 2...
متن کاملNeutral and DEAE dextrans as tracers for assessing lung microvascular barrier permeability and integrity.
Steady-state lymph-to-plasma concentration ratios (L/Ps) of neutral dextrans, cationic DEAE dextrans, and endogenous proteins were determined under normal and increased permeability conditions in six unanesthetized yearling sheep prepared with chronic lung lymph fistulas. Fluorescent dextrans with radii ranging from 1 to 30 nm were intravenously infused, and after 24 h, perilla ketone (PK) was ...
متن کاملThe Permeability of Glomerular Capillaries to Graded Dextrans
Graded dextrans have been used as tracers to identify the primary permeability barrier(s) to macromolecules among the structural elements (endothelium, mesangium, basement membrane, epithelium) of the glomerular capillary wall. Three narrow-range fractions of specified molecular weights and Einstein-Stokes radii (ESR) were prepared by gel filtration: (a) 32,000 mol wt, ESR = 38 A; (b) 62,000 mo...
متن کاملA Study of Capillary Permeability in Experimental Burns and Burn Shock Using Radioactive Dyes in Blood and Lymph.
The integrity of the capillary membrane is essential to the maintenance of the circulation in a normal animal. Increased loss of plasma colloids through the capillary wall may be an important factor in the development of a pathologic state. In recent years, interest has centered upon abnormal capillary permeability, and local changes in permeability have been demonstrated in areas of trauma and...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Journal of Cell Biology
دوره 50 شماره
صفحات -
تاریخ انتشار 1971